Extended Data Fig. 2: Increased proportion of auxotrophic subpopulations in SeMeCos are independent of drug effects on plasmid segregation or stability.
(a) Drug disk diffusion assays of wild-type (WT) strains without plasmid or transformed with MitoLOC plasmid2 that encode for nourseothricin resistance (NAT) compared against the SeMeCo strain which carries 4 plasmids when exposed to uniconazole. (b) Disk diffusion assays of WT prototrophic strain compared against singly auxotrophic strains and quadruple auxotrophic parental strain when exposed to miconazole. SM indicated minimal media condition versus SM + HLUM whereby minimal media was supplemented with the 4 amino acids. Singly auxotrophic strains were supplemented with the respective amino acid to ensure their growth in the absence of genomic or plasmid complementation.
