Extended Data Fig. 4: Prototrophs present growth advantage relative to co-growing auxotrophs. | Nature Microbiology

Extended Data Fig. 4: Prototrophs present growth advantage relative to co-growing auxotrophs.

From: Microbial communities form rich extracellular metabolomes that foster metabolic interactions and promote drug tolerance

Extended Data Fig. 4

(a) pHLUM strain carrying a single plasmid complementing the 4 auxotrophies. Loss of the plasmid results in immediate reduction in production capacity for HLUM opposed to sequential loss in SeMeCos. (b) Competitive growth assay in which the pHLUM strain was co-cultured with the SeMeCo strain. Over 18 days, replated every 48 h, in 3 independent cultures (A, B and C), the pHLUM strain retaining full prototrophy, slowly outcompetes all SeMeCo derived subpopulations in terms of growth.

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