Fig. 2: Infectivity and replication of SARS-CoV-2 variant Lambda.
a, Levels of infection of SARS-CoV-2 pseudotypes bearing the Alpha (B.1.1.7), Gamma (P.1), Lambda (C.37) and Delta (B.1.617.2) spike protein in HEK293T cells stably expressing ACE2. Cells were seeded in 96-well plates and infected with 0.5 RT units of the indicated pseudotyped viruses for 48 h. Two biological replicates were assessed in three independent experiments. Data are presented as the mean ± s.d. of three independent experiments. P values were determined by ANOVA. b, Genomic RNA levels Alpha, Gamma, Lambda and Delta viruses produced on infection of Vero-E6 cells. Cells were infected at a MOI = 1. Genomic RNA levels are expressed as cycle threshold (Ct) values, which are inversely related to viral load. Data are presented as the mean ± s.d. of three experiments. P values were determined by ANOVA from a sample size of n = 3. c, Levels of infection of SARS-CoV-2 pseudotypes bearing the Lambda (C.37) and Lambda mutant T859 spike protein in HEK293T-ACE2 cells. Cells were seeded in 96-well plates and infected with 0.5 RT units of the indicated pseudotyped viruses for 48 h. Two biological replicates were assessed in three independent experiments. Data are presented as the mean ± s.d. of three independent experiments. P values were determined by Mann–Whitney signed-ranked test.
