Extended Data Fig. 6: Multiple sequence alignment of betacovronaviruses around F592 and T859.

(a) Insight on the neighborhood of T859 at the interface of two spike protomers. The inset on the right shows the hydrophobic interaction with F592 in the next protomer. G164 is also shown for reference. (b) The hydrophobic interaction between T859 and F592 is only possible in Sarbecovirus as other subgenera present a putatively correlated mutation at those positions (K and S, respectively).