Extended Data Fig. 1: Transfected populations efficiently incorporate a variety of HiT vectors. | Nature Microbiology

Extended Data Fig. 1: Transfected populations efficiently incorporate a variety of HiT vectors.

From: Screening the Toxoplasma kinome with high-throughput tagging identifies a regulator of invasion and egress

Extended Data Fig. 1

a, Fluorescence microscopy of the tagged populations displaying the correct localization of each kinase and expression levels consistent with flow cytometry (Fig. 1b). b, Live microscopy of V5-T2A-mKate2 HiT-tagged population (merged image in Fig. 1g). c, Immunofluorescence microscopy of population tagged with the HA-U1 HiT vector following treatment with rapamycin or vehicle control (merged image in Fig. 1i). d, Flow cytometry of parasite populations tagged with the V5-mNG-mAID HiT vector targeting CDPK1 or CDPK3 and treated with either IAA or vehicle control for 24 h (excerpt shown in Fig. 1l).

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