Extended Data Fig. 6: In vitro cytotoxicity of Y180 and in vivo toxicity study of Y180 and (S)-6c.
(a-d) Cells were treated with 5-fold serial diluted Y180 for 24 hours (a and c) or 72 hours (b and d) before cell viability was measured in (a and b) Calu3 and (c and d) Vero E6-TMPRSS2. Luciferase readings were normalized to mock treatment controls. Data are shown as mean ± SD, n = 6 biological replicates. e. Body weight change (percentage of initial body weight) with Y180 treatment (400 mg/kg/dose, twice per day) via oral delivery route for one week in rats (n = 6). f. Representative images of H&E staining of heart, liver, spleen, lung and kidney of rats orally treated with vehicle, Y180 and (S)-6c. Three randomly selected rats were sampled in each group were used for histology analysis. Scale bar represents 100 μm. CC50, 50% cytotoxicity concentration.
