Extended Data Fig. 4: Purification and identification of herbicolin A.

a, Comparative total ion flow diagram profile between ZJU23 and deletion mutants in the HA biosynthetic gene cluster. b, High-resolution mass spectra (HRMS) of herbicolin A showing a peak at m/z 1300.7378, which corresponds to the [M+H] + ion and another at m/z 650.8722, which corresponds to the [M+2H]2+ ion. c, 1H NMR spectrum of HA. d, NOESY NMR spectrum. e, TOCSY NMR spectrum. f, 1H-13C HSQC NMR spectrum. g, 1H-13C HMBC NMR spectrum.