Extended Data Fig. 9: Humanized mouse model for HCMV infection.
(a) The experimental workflow of in vivo HCMV infection experiment in humanized mice: NOD-scid IL2RGγcnull mice were engrafted with human CD34+ HPCs and then transfused with NHDFs infected with HCMVWT, HCMV△UL37x1, HCMV△2-23 or HCMVK40R (n = 6/group). Uninfected mice served as the negative control group (n = 6). At 28 days post infection, in each group, three mice were euthanized for tissue harvesting and three mice were treated with G-CSF and AMD-3100 for another 10 days and then euthanized for tissue harvesting and analysis. (b-c) NHDF cells were infected with wild-type or mutant HCMV. At 12 h.p.i., the mRNA levels of the indicated HCMV genes in NHDFs were measured by qPCR (b) and the protein level of HCMV-expressed reporter GFP was measured by immunoblot (c). Immunoblots are representative of three independent experiments. Graphs in b show mean ± SD (n = 3, biologically independent experiments). Statistical significance was determined by one-way ANOVA.
