Extended Data Fig. 2: UL37x1 inhibits the DNA-triggered innate immune signaling in 293 T cells.
(a) Luciferase reporter assay analyzing IFNβ, ISRE, or NF-κB promoter activity was conducted in 293 T cells transfected with the plasmid of FLAG-cGAS plus FLAG-STING or an empty vector together with the indicated amounts of FLAG-UL37x1 expression plasmid for 24 h. The lower blots showed the expression levels of these transfected proteins. (b) 293 T cells were transfected with the plasmid of FLAG-cGAS plus FLAG-STING or an empty vector together with the indicated amounts of FLAG-UL37x1 expression plasmid for 24 h, followed by qPCR of the indicated antiviral genes. The lower blots showed the expression levels of these transfected proteins. (c) 293 T cells stably expressing STING were transfected with the plasmid of FLAG-UL37x1 or an empty vector. After 24 h, cells were untreated (mock) or transfected with indicated DNA ligands (HSV60, DNA90, or HSV120). At 3 and 6 h post transfection (h.p.t.), total RNA was extracted and subject to qPCR of indicated antiviral genes. Graphs show mean ± SD (n = 3, biologically independent experiments). Statistical significance was determined by two-way ANOVA. Immunoblots are representative of three independent experiments.
