Extended Data Fig. 3: UL37x1 inhibits TBK1-mediated immune signaling.
(a) 293 T cells were transfected with the indicated plasmid in the presence or absence of the plasmid of FLAG-UL37x1. At 24 h.p.t., qRT-PCR was performed to measure the transcription of indicated antiviral genes. (b) HFFs were infected with HCMV for indicated time, followed by qPCR analysis of UL37x1. (c-d) HFFs were transfected with siRNA specifically for UL37x1 (UL37x1-siRNA#1, UL37x1-siRNA#2, or UL37x1-siRNA#3) or a control siRNA (Control). After 24 h, cells were infected with HCMV. At 6 h.p.i., qPCR was performed to measure the transcription of UL37x1 (c). At 12 h.p.i., cell lysates were subject to immunoblots with indicated antibodies (d). Immunoblots are representative of three independent experiments. Graphs show mean ± SD (n = 3, biologically independent experiments). Statistical significance was determined by two-way ANOVA in a, or one-way ANOVA in c.
