Extended Data Fig. 7: Effects of experimental warming on sporulation families or genes of Firmicutes and Actinobacteria.
From: Reduction of microbial diversity in grassland soil is driven by long-term climate warming
a, Number of Firmicutes families whose relative abundances increased, decreased or unchanged under warming. b, Number of Actinobacteria families whose relative abundances increased, decreased or unchanged under warming. Significant changes (p < 0.05) are based on Wald type II χ² tests (n = 360 independent soil samples) of the warming effects in linear mixed-effects models (relative abundance ~ warming × precipitation level × clipping + (1|Block) + (1 | year)). ‘Yspore’, ‘Nspore’, and ‘NAspore’ refer to known spore-formers, known non-spore-formers, and information not available on the ability to form spores, respectively. The spore-forming capability or different families within Firmicutes and Actinobacteria were mainly based on databases and literature. c, Effect sizes of experimental warming on the (rescaled) relative abundance of major sporulation genes in Firmicutes (spo0A gene) and Actinobacteria (bldD gene) based on linear mixed-effects models. The sporulation genes were retrieved from shotgun sequencing data. Data are presented as mean values ± standard errors of the estimated effect sizes. Statistical significance is based on Wald type II χ² tests (n = 64 independent soil metagenome samples).
