Extended Data Fig. 3: Sequencing depth among different treatments for the bacterial community (a), fungal community (b), and protistan community (c).
From: Reduction of microbial diversity in grassland soil is driven by long-term climate warming
A total of 360 soil samples over 8 years were analyzed with 16S ribosomal RNA (rRNA) gene for bacteria and archaea, the internal transcribed spacer (ITS) between 5.8S and 28S rRNA genes for fungi, and the 18S rRNA gene for protists. For 18S sequences, only those annotated as protists were selected for the subsequent analyses. An average of 56,182 ± 27,613, 23,569 ± 16,323, and 11,146 ± 10,528 sequence reads were obtained for bacteria, fungi, and protists, respectively. There was no significant difference between treatments in the number of sequences (that is, sequencing depth) for the bacteria, fungal, and protistan communities except half precipitation (H), double precipitation (D), clipping (C), and double precipitation & clipping (DC) for protists (p = 0.002-0.021). Groups: Control (N), Warming (W), Half precipitation (H), Double precipitation (D), Clipping (C), Warming & Half precipitation (WH), Warming & Double precipitation (WD), Warming & Clipping (WC), Half precipitation & Clipping (HC), Double precipitation & Clipping (DC), Warming & Half precipitation & Clipping (WHC), and Warming & Double precipitation & Clipping (WDC). In the box plots, hinges show the 25, 50, and 75 percentiles. The upper whisker extends to the largest value no further than 1.5 * IQR from the upper hinge, where IQR is the inter-quartile range between the 25% and 75% quartiles; the lower whisker extends to the smallest value at most 1.5 * IQR from the lower hinge.
