Extended Data Fig. 4: Rarefaction curves.
From: Reduction of microbial diversity in grassland soil is driven by long-term climate warming
The number of ASVs with an increasing number of sequences (a, c, e) and accumulation curves of the number of ASVs with an increasing number of samples (b, d, f) for the bacterial community (a, b), fungal community (c, d), and protistan community (e, f). The observed number of ASVs with warming treatment was lower compared with all those without warming treatment except warming & double precipitation & clipping (WDC) versus double precipitation & clipping (DC) for fungi and warming & clipping (WC) versus clipping (C) for protists in (a, c, e) (Paired t test, p < 0.0001). The number of samples did not have a substantial influence on the differences between warming and non-warming control as shown in (b, d, f). After removing global singletons and resampling, the rarefaction curves approached asymptotes for all treatments, indicating that the sequencing depth was sufficient for assessing the effects of various climate change factors on the diversity of these soil microbial communities.
