Extended Data Fig. 10: Peptide809-833 binding mAbs 555D6 and 125C1 could not neutralize SARS-CoV-2 pseudovirus.
From: Neutralization mechanism of a human antibody with pan-coronavirus reactivity including SARS-CoV-2
a, ELISA binding activities of 9 isolated S2 targeting antibodies to peptide809-833. Data are shown as mean value of duplicates. b-c, Neutralizing activities of 555D6 (b) and 125C1 (c) against SARS-CoV-2 pseudovirus. Results are depicted as the mean ± s.d. n = 3. d-e, ELISA-based epitope alanine scanning of 555D6 and 125C1 on the peptide809-833 of SARS-CoV-2, shown as EC50 (ng/ml). Non-binding EC50 were set to over 10,000 ng/ml. f, The EC50 fold change of 555D6 and 125C1. The EC50 fold change was the ratio of EC50 of antibodies against mutants compared with wildtype peptide. g, 125C1 and 555D6 could not block the trypsin-induced S2’ cleavage. 76E1 was included as a positive control. Representative data were shown from two independent experiments (a-g).
