Extended Data Fig. 7: BBV152 vaccination generates multifunctional spike-specific CD4+ T-cell response with the polarization towards Th1-cell phenotype.
Cytokine levels were measured by multiplex bead-based immunoassay in the cell culture supernatants of PBMCs stimulated or not with spike peptideMP for 24 h, and compared between BBV152 vaccination (n = 23) and recovery from mild COVID-19 (n = 20). The standard curve depicting the fluorescence intensity (FI) of cytokine-standard and the adjacent bar graph showing the concentration (pg/ml) of T-cell specific cytokines in vaccination and infection for (a) IFN-γ (b) IL4 (c) IL17 (d) IL13 (e) IL2 and (f) TNF-α. (g) Stacked graph showing the comparison of mean concentration of each cytokine in BBV152 vaccination and recovery from mild COVID-19. The data denotes the mean±s.e.m. The positive response was determined as the response over Lower Limit of Quantification (LLOQ) and statistical significance in spike peptideMP stimulation over unstimulated conditions. Statistics by two-tail Mann-Whitney test. ns: non-significant.
