Extended Data Fig. 5: Comparison of AIM and ICS assay for the quantitative measurement of SARS-CoV-2 specific CD4+ T cells. | Nature Microbiology

Extended Data Fig. 5: Comparison of AIM and ICS assay for the quantitative measurement of SARS-CoV-2 specific CD4+ T cells.

From: Inactivated whole-virion vaccine BBV152/Covaxin elicits robust cellular immune memory to SARS-CoV-2 and variants of concern

Extended Data Fig. 5

To validate quantification of SARS-CoV-2 specific T cells by AIM assay, IFN-γ intracellular cytokine staining (ICS) and AIM was performed on the PBMCs after stimulation with ancestral SARS-CoV-2 spike MPs for 24 hours at 37 °C. In the Activation-induced markers (AIM) assay, antigen-specific CD4+ T cell were quantified as a percentage population of AIM+ (OX40+CD137+) CD4+ T cells. In the IFN-γ ICS assay, antigen-specific CD4+ T cell were quantified as a percentage population of IFN-γ+CD4+ T cells. The graph depicts the correlation between SARS-CoV-2 spike-specific AIM+ CD4+ T cells and IFN-γ+ CD4+ T cells measured in respective PBMCs (n = 8) from BBV152 vaccinated and COVID-19 recovered individuals. Statistics by two-tail Pearson correlation coefficient.

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