Extended Data Fig. 5: Subtomogram averages of the P. falciparum RSA. | Nature Microbiology

Extended Data Fig. 5: Subtomogram averages of the P. falciparum RSA.

From: Rhoptry secretion system structure and priming in Plasmodium falciparum revealed using in situ cryo-electron tomography

Extended Data Fig. 5

(a) Schematic of the RSA, apical vesicle, and PPM demonstrating the top view and side view orientations used to generate 2-D slices of the subtomogram averages. (b, c) Side views (b) and top view (c) of the subtomogram average of the RSA-A structure before applying 8-fold symmetry. Left to right panels: from peripheral to central cross sections (b) or from extracellular to intracellular cross sections (c). (d–g) Side views (d, f) and top view (e, g) of the subtomogram average of the RSA-2R structure, before and after applying 8-fold symmetry. (h–k) Side views (h, j) and top views (i, k) of the subtomogram average of the RSA-1R structure, before and after applying 8-fold symmetry. (l) Gold standard Fourier shell correlation plot of the final, 8-fold symmetrized subtomogram averages of the three RSA structures. Scale bars = 10 nm.

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