Extended Data Fig. 3: Gel electrophoresis of FRR1, URA5, and FUR1 PCR products from resistant colonies. | Nature Microbiology

Extended Data Fig. 3: Gel electrophoresis of FRR1, URA5, and FUR1 PCR products from resistant colonies.

From: Uncontrolled transposition following RNAi loss causes hypermutation and antifungal drug resistance in clinical isolates of Cryptococcus neoformans

Extended Data Fig. 3

Gel electrophoresis of FRR1 PCR products from (A) all H99 R + FR colonies and a subset of (B) Bt65 and Bt81 R + FR colonies sequenced in Fig. 1D. PCR amplification of wild-type FRR1 in C. neoformans produces a 1,165 bp electrophoretic species (primers ZC7/8). Gel electrophoresis of a subset of (C) URA5 PCR products from H99, Bt65, and Bt81 5-FOAR colonies and (D) FUR1 and (E) UXS1 PCR products from 5-FCR colonies of Bt65 and Bt81.

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