Extended Data Fig. 1: Binding of nucleotides and dinucleotides to BsIMPDH determined by isothermal titration calorimetry (ITC).
From: Diadenosine tetraphosphate regulates biosynthesis of GTP in Bacillus subtilis
a-j. A 25 µM concentrated solution of purified BsIMPDH was titrated with a. AMP, b. ADP, c. ATP, d. Ap3A, e. Ap4A, f. Ap5A, g. Ap6A, h. Ap3G, i. Ap4G, or j. Ap5G. The differential power (DP, upper plot) for each injection of ligand was recorded and used to determine the dissociation constant Kd from a fitting of the calculated binding enthalpies (ΔH, lower plot).
