Fig. 5: B. thetaiotaomicron sulfotransferase activity influences host CholSulf and cholesterol levels in a gnotobiotic mouse model.
From: Characterization of interactions of dietary cholesterol with the murine and human gut microbiome
a,b, Expression of Bt_0412 (a) and Bt_0416 (b) from B. thetaiotaomicron cultured in 10% tryptone, followed by treatment with vehicle (Veh), lactose (Lac) or cholesterol (Chol) (n = 6 per condition). c–f, Targeted metabolomics performed on GF mice, GF mice monocolonized with B. thetaiotaomicron harbouring either BT_0416-competent (WT: BtΔtdk) or -null (KO: BtΔtdkΔ0416) strains, and CONV-D mice (n = 10 per condition). CholSulf measurements from caecal content (c), stool (d) and whole blood (e) of GF, KO, WT and CONV-D mice. f, Serum total cholesterol measurements of GF, KO, WT and CONV-D mice. Bar chart values are mean ± s.d. and statistical analyses were performed using one-way analysis of variance with Tukey’s multiple comparison correction. NS, P > 0.05; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.
