Extended Data Fig. 2: Development of FACS gates for detecting and isolating cholesterol-interacting gut microbiota using Eubacterium coprostanoligenes.
From: Characterization of interactions of dietary cholesterol with the murine and human gut microbiome
a, Fecal microbes were freshly extracted from murine faecal pellets, spiked with E. coprostanoligenes cultured with CholAlk and click-stained with AF647-azide (E. copAlk). b, Illustration of the FACS gating strategy aiming to isolate cholesterol-interacting gut microbes from complex gut microbiome populations. Representative scatter plots of different ratios of spiked-in E. copAlk to faecal microbes validate the gating stringency suitable for downstream FACS application on the in vivo microbial samples.
