Extended Data Fig. 8: mEV-A71/CG-48/A+ elicits protective antibodies in ZAP+/+mice.
From: Rational attenuation of RNA viruses with zinc finger antiviral protein
(a) Neutralizing activity in plasma from mice after mEV-A71/CG-48/A+ infection (n = 4, at day 5 of age, blood collection at 6-weeks) or mock-infected mice (n = 3) evaluated using EV-A71 NanoLuc luciferase reporter virus. 293T cells were infected with the antibody:virus mixture, incubated for 48 h and luciferase activity was measured. Mean and SD plotted. (b) Clinical score and survival probability following mEV-A71 (wildtype) infection of the offspring of ZAP+/+IFNAR−/− females previously inoculated with mEV-A71/CG-48/A+ (n = 17 pups from 3 different females) or mock-infected (n = 7 pups from 1 female) at day 5 of age. Clinical score and survival were assessed daily until weaning age. Mean and SEM plotted.
