Extended Data Fig. 9: Colonization of Veillonella in a mouse model of colitis.
(a) Raw Ct values of bacterial 16 S rRNA gene measured by qPCR in the stool of WT-colonized ASF mice with and without DSS treatment (n = 8–11 mice). (b) Absolute abundance of V. parvula in the stool of ASF mice as measured by CFU per mg of stool on selective SKLN media (n = 3 mice). (c) Absolute abundance of V. parvula in the colonic tissue of ASF mice as measured by CFU per mg of tissue on selective SKLN media (n = 4 mice). (d) Concentration of lactate in the gut of control- and DSS-treated ASF mice colonized with V. parvula (n = 8 mice). (e) Relative expression of the IIdD, lldP, hisA and argB genes in WT Veillonella colonizing the stool of mice with and without DSS treatment (n = 3–4). The V. parvula gyrA gene was used as an internal control. (f) Colon lengths of WT and iNOS KO SPF mice (n = 4–7). Data represent two independent experiments and show mean (a, d–f) ± SEM (d–f) or median (b, c) values. Data were analyzed by one-way ANOVA and Tukey’s HSD test or Mann-Whitney U-test.
