Extended Data Fig. 3: Nitrate metabolism in Veillonella.
(a) Levels of nitrate and nitrite were measured at the mid-exponential phase in cells growing on SKN medium supplemented or not with lactate as the carbon source (n = 3 biological replicates). The measured concentrations of nitrate and nitrite in the uninoculated SK medium were 43.161 ± 0.410 mM and <0.050 mM, respectively (n = 3 biological replicates). (b) Ammonium (n = 6 biological replicates) or (c) nitrite (n = 4 biological replicates) were used to determine if the positive effect of nitrate on growth of V. parvula was due to nitrate being used as a source of nitrogen or an electron acceptor, respectively. Growth rates (µmax) were calculated for each growth curve. Data represent mean ± SEM, analyzed by one-way ANOVA and Tukey’s HSD test (a, b) or Dunnett’s test (c). NS, not significant.
