Extended Data Fig. 7: Supporting data for neuraminidase experiment.
From: Coinfection by influenza A virus and respiratory syncytial virus produces hybrid virus particles
(a) Schematic demonstrating experimental design. (b) NA-treated and untreated cells stained with Maackia Amurensis Lectin II (MAL II) in yellow (top row) and Erythrina Cristagalli Lectin (ECL) in cyan (bottom row). Scale bar represents 20 µm. Images representative of n = 2 biologically independent experiments. (c) Viral input in pfu/ml of IAV as determined by back-titration of inoculum for NA-experiment by IAV plaque assay. (d) Ratio of RSV entry into NA-treated cells versus control cells when harvested from single infection (green bars) or mixed infection (blue bars). RSV entry to NA-treated cells was calculated as a percentage of the RSV-positive cell count in the matched untreated control. (e) Ratio of virus entry of IAV only or RSV only (red bars) into NA-treated over control cells, compared to entry of IAV pre-mixed with RSV or RSV pre-mixed with IAV into NA-treated over control cells (blue bars). Data shown in (c-e) was collected from n = 2 (c) or 3 (d-e) independent experiments and statistical significance calculated by two tailed Mann Whitney test, ns indicates p > 0.05. Bars represent mean and data points represent biological replicates.
