Extended Data Fig. 1: Vectors used to determine the minimal gene set of the Variovorax paradoxus CL14 IAA degradation locus required to degrade IAA.
From: Diverse MarR bacterial regulators of auxin catabolism in the plant microbiome
a) Vectors, named at left, contain the genes indicated by the blue bar in broad host range vector pBBR1MCS-2. For the vector insert, the Variovorax iad locus genes were cloned in the place of lacZ on pBBR1MCS-2 with the start codon of the first gene from the insert in place of the start codon of lacZ. The vector inserts contain the intergenic region following the last gene of the insert, ending at the start codon of the next sequential gene not contained in the insert. Vector 2, which contains part of gene 72 through part of gene 61, was identified previously14 from a library of random clones as being able to confer IAA degradation ability on Escherichia coli and Acidovorax sp. Root219. b/c) IAA degradation in E. coli NEB 10beta containing the indicated vectors after 3 days of culture in 2xYT medium supplemented with IAA (n = 3 biological replicates, bar represents the average, error bars represent one standard deviation from the average).
