Extended Data Fig. 2
a. GAPDH-normalised TRIM5 mRNA levels, measured after transfection of THP-1 treated with non-targeting CRISPR (NT-KO) or cGAS CRISPR (cGAS-KO) and transfected with TRIM5 targeting siRNA (siTRIM5) or non-targeting control siRNA (siCtrl). N = 3 independent experiments. b. Anti-MAVS or tubulin western blot of non-targeting KO cells (NT-KO) or MAVS KO cells. c. % infection of HIV-1(M), HIV-2 and HIV-1(O) in NT-KO or MAVS KO cells. d. IFIT1 reporter activation after viral infection normalised against mock infected. e. GAPDH-normalised mRNA levels expressed as fold induction over mock-treated non-targeting KO control (NT-KO) or MAVS KO THP-1 cells 24 h post-infection or after poly I:C transfection (500 ng/mL). Mean + /− SD, n = 2 independent experiments.
