Fig. 3: Comparison of LbuCas13a anti-phage activity across dsDNA E. coli phage phylogeny.
From: Broad-spectrum CRISPR-Cas13a enables efficient phage genome editing
a, Network graph representation of E. coli phages and their relatives. Nodes represent phage genomes that are connected by edges if they share significant similarity as determined by vContact276 (protein similarity). Nodes are shaded red if they are classified as an E. coli phage and blue if they only share similarity. Nodes are shaded black if they were assessed for sensitivity to LbuCas13a. b, EOP experiments for Cas13a designed to target an early or late transcript. EOP values represent the average of three biological replicates for a single crRNA compared to an RFP-targeting negative control crRNA. Phages T4, EdH4, λ, T5 and T7 have additional crRNAs that were tested and are presented in Supplementary Figs. 2, 8, 10, 14 and 15, respectively.
