Fig. 2: Factors shaping the tick virome.
From: Metavirome of 31 tick species provides a compendium of 1,801 RNA virus genomes
a, Between-group clustering of viromes among ticks in the family Argasidae and six genera of the family Ixodidae by t-SNE analysis. b, Shannon indexes of α diversity of tick viromes. Statistical significance was determined by two-sided Kruskal–Wallis test for multiple comparison, with P values adjusted by the Bonferroni method. c, Shannon indexes of tick viromes were compared between areas with two or more tick species and those with single tick species within a radius of 50 km. The P value was determined by two-sided Mann–Whitney U test. d, Maximum-likelihood phylogeny of genetic populations for Dermacentor silvarum and Rhipicephalus microplus. Lineages of each tick species were classified on the basis of nuclear single-nucleotide polymorphisms from the transcriptome data. Bootstrap values above 0.8 are indicated by grey asterisks. The outer colour of dots represents virome diversity: red, higher Shannon index; blue, lower Shannon index. The inner colour of dots indicates the location of the tick collection. e, Shannon indexes of tick viromes were compared between fed and unfed ticks of Haemaphysalis, Ixodes and Rhipicephalus genera. The indexes were compared between female and male ticks of the genus Rhipicephalus. The P value was determined by two-sided Mann–Whitney U test. Sample numbers are marked in brackets. Boxplot elements: centre line, median; box limits, upper and lower quartiles; whiskers (error bars), the highest and lowest points within 1.5× interquartile range of the upper and lower quartiles.
