Extended Data Fig. 7: AMFR interacts with TAB3 and recruits TAB3 to the ER. | Nature Microbiology

Extended Data Fig. 7: AMFR interacts with TAB3 and recruits TAB3 to the ER.

From: Staphylococcal virulence factor HlgB targets the endoplasmic-reticulum-resident E3 ubiquitin ligase AMFR to promote pneumonia

Extended Data Fig. 7

(a) Coimmunoprecipitation and immunoblot analysis of HEK293T cells co-transfected with MYC-TAB3, plus FLAG-AMFR, and FLAG-AMFR mutant vectors. (b) Coimmunoprecipitation and immunoblot analysis of HEK293T cells co-transfected with FLAG-TAB3, plus MYC-TAB3, and MYC-TAB3 mutant vectors. (c) Confocal microscopic imaging of HeLa cells expressing MYC-TAB3 (green) and FLAG-AMFR or FLAG-AMFR mutant vectors (red). Blue indicates ER-Tracker stain of ER. Yellow in merge 1 indicates the co-localization of TAB3 and AMFR. White in merge 2 indicates the co-localization of TAB3, AMFR, and ER. Scale bar, 10 mm. (d) Immunoblot analysis of TAB3 expression in cytoplasm and light membranes (LM, containing ER) of Amfr−/− BMDMs transfected with AMFR, or AMFR mutants infected with MRSA43300. (e) Immunoblot analysis of TAB3 expression in cytoplasm and light membranes (LM, containing ER) of Tab3−/− BMDMs transfected with TAB3, or TAB3 mutants infected with MRSA43300. Data shown are representative of three independent experiments.

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