Extended Data Fig. 10: Staphylococcus aureus g-hemolysin B (HlgB) plays a critical role in AMFR-mediated pneumonia. | Nature Microbiology

Extended Data Fig. 10: Staphylococcus aureus g-hemolysin B (HlgB) plays a critical role in AMFR-mediated pneumonia.

From: Staphylococcal virulence factor HlgB targets the endoplasmic-reticulum-resident E3 ubiquitin ligase AMFR to promote pneumonia

Extended Data Fig. 10

(a) Hematoxylin and eosin staining of lung tissues from indicated mice after MRSA USA300 or USA300∆hlgB exposure (n = 5 per group). (b) Lung injury was assessed by histological scores in different groups. (c) Neutrophils (Neu., Ly-6G+) and alveolar macrophages (AMs, CD11c+ F4/80+) in BALF were analyzed using flow cytometry. (d) Total cell, neutrophils, and alveolar macrophages counts in BALF were estimated. (e) BALF protein were measured. (f) Bacterial burden in lung tissues from Amfrfl/fl and LysM-Cre Amfrfl/fl mice intratracheally injected with USA300 or USA300∆hlgB for the indicated times. Data are representative of three independent experiments (means ± SD) (n = 5 mice per group per experiment), two-way ANOVA (Sidak’s test).

Source data

Back to article page