Extended Data Fig. 3: AMFR deficiency in macrophages ameliorates intracellular MRSA-induced pneumonia. | Nature Microbiology

Extended Data Fig. 3: AMFR deficiency in macrophages ameliorates intracellular MRSA-induced pneumonia.

From: Staphylococcal virulence factor HlgB targets the endoplasmic-reticulum-resident E3 ubiquitin ligase AMFR to promote pneumonia

Extended Data Fig. 3

(a) Gating strategy for flow cytometry analysis of the neutrophils (Neu., Ly-6G+) and alveolar macrophages (AMs, CD11c+ F4/80+) in the BALF of MRSA-infected mice. (b) BALF cells were sorted by Flow cytometry following Cytospin preparations and Giemsa staining. Representative neutrophils (Neu.) and alveolar macrophages (AMs) are shown. (c) BALF neutrophils and AMs’ ratios were estimated. Data shown are representative of three independent experiments, or presented as means ± SD from three independent experiments (n = 5 mice per group per experiment), two-way ANOVA (Bonferroni test).

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