Extended Data Fig. 8: Gating strategy for flow cytometry analysis. | Nature Microbiology

Extended Data Fig. 8: Gating strategy for flow cytometry analysis.

From: A MOPEVAC multivalent vaccine induces sterile protection against New World arenaviruses in non-human primates

Extended Data Fig. 8

The gates used to quantify IFNγ-producing and CD154-expressing T cells are presented for an unstimulated sample (a) and for the same sample stimulated with staphylococcus-enterotoxin A (SEA), as a positive control (b). FSCint/FSCtof was used to select singlets (singlets gate). Then, dead cells are excluded using live-dead staining (live gate). Lymphocytes were selected using FSCint/SSCint parameters (LC gate). Then, CD4+ and CD8+ T cells were selected using CD3/CD4 and CD3/CD8 staining (CD4+ and CD8+ gates). Finally, the percentage of IFNγ-producing and CD154-expressing CD4+ and CD8+ T cells is determined using a quadrant in the IFNγ/CD154 dotplot. c. A similar strategy was applied for CD137 and GrzB detection.

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