Extended Data Fig. 9: Activation of T cells in response to peptide stimulation. | Nature Microbiology

Extended Data Fig. 9: Activation of T cells in response to peptide stimulation.

From: A MOPEVAC multivalent vaccine induces sterile protection against New World arenaviruses in non-human primates

Extended Data Fig. 9

a. PBMCs sampled at days 14 and 24 post-prime and day 19 post-boost were stimulated with overlapping peptides covering MACV NP and GP and LASV strain Josiah NP. SEA was used as a positive control. After an overnight incubation, the cells were stained with conjugated antibodies and analyzed by flow cytometry for the expression of CD154, CD137, GrzB and IFNγ. Expression values represent the difference between stimulated and non-stimulated cells. Light blue dots represent animals vaccinated with a prime-boost strategy (n = 4, except for J19 boost where SEA n = 2, NP LASV n = 3) and black dots the control animals (n = 3). The dots were not separated when the expression values were close to 0. b. After challenge, peptide stimulation was performed on whole blood. GPC and NP specific T cell responses were evaluated. The difference from the non-stimulated condition is represented (Ctrl: n = 3, Vacc: n = 4). The SEA control at day 0 is presented for comparison.

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