Extended Data Fig. 1: GPs expression by MOPEVAC viruses.
a. VeroE6 cells were infected at a moi of 0.001 and cellular RNAs were extracted at day 0 and day 3 post-infection. The ratio of GPC expression of day 3 relative to day 0 was calculated and represented for each MOPEVAC virus. b. Expression of GP2 detected by KL-AV-2A149 antibody. GP2 protein expression was detected by western blot from 105 ffu of MOPEVAC viruses, in a single experiment. The antibody has been described to detect JUNV, GTOV and MACV but its binding on CHAV and SABV was not known. These results show the expression by the different MOPEVAC viruses of the GP2 proteins of all NWA except the one of SABV, probably because of a lack of cross-reactivity of the antibody.
