Extended Data Fig. 8: Histopathological analysis of the infection of SARS-CoV-2 B.1.1.7 strain in C57BL6 mice.
From: Fluorogenic reporter enables identification of compounds that inhibit SARS-CoV-2
(a) H & E staining of lung sections from the infected mice. C57BL6 mice were infected by the intranasal route with 105 pfu of SARS-CoV-2 (B.1.1.7). Bruceine A and gamabufotalin were used as 3.25 mg/kg by intranasal inoculation at both 24- and 48-h post-infection. At day 3 (that is, 3 days post-infection), lungs were harvested and fixed in 4% PFA and embedding into paraffin and cut at 5 μm slides, stained with hematoxylin and eosin (H & E). Black boxes point to regions of the lung anatomy where inflammation was assessed. The magnified images corresponding to the boxed areas are shown on the right panels. Regions where inflammation was detected are indicated by arrows. Scale bar: 200 μm. (b) Pathological scores of the lung sections. Five mice in each group were analyzed using a semi-quantitative method described in the methods. The dotted black line represents pathology score = 0. Data are shown as mean ± s.d. P value, two-sided non-paired t-test between treated and vehicle groups. *P < 0.05. Exact P values: bruceine A 0.046, gamabufotalin 0.046.
