Fig. 4: Cell-cycle-dependent organelle composition identifies division and morphogenesis factors.
From: Genome-wide subcellular protein map for the flagellate parasite Trypanosoma brucei
a, Six examples of proteins that were found more strongly in the new copy of an organelle, showing cells towards the end of the cell cycle (2K1N and 2K2N). BB, basal body. b, Six examples of proteins that were found more strongly in the old copy of organelles, showing cells towards the end of the cell cycle. c, Examples of proteins localizing to different spindle structures. d, Both proteins localizing to the spindle with predicted transmembrane domains. e, Thin-section transmission electron micrographs of the nucleus of parental in comparison with Tb927.3.3500 (CMP1) deletion mutant cells showed abnormal intranuclear membrane never seen in parental cells (arrows) in 42% of nuclei, n = 38 cells from one clonal cell line. f, CPC1::mNG localization in cytokinetic cells in parental in comparison to CMP1 deletion mutant cells shows the normal cleavage furrow (f) signal and an abnormal additional cytoplasmic point never seen in parental cells (S, probably the mid-spindle remnant) in 76% of cytokinetic cells, n = 17 cells from one clonal cell line. g, Localization of the proteins which localize to both the spindle pole (spindle nucleating) and basal body or pro-basal body (axoneme nucleating). GCP4 was not detectable at the spindle pole but was included for completeness of the gamma tubulin ring complex. h, Localization of the six proteins that localize to both the basal body or pro-basal body (axoneme nucleating) and the cell anterior tip (hypothetically cortical cytoskeleton nucleating) and not to other structures. i, Euler diagram of proteins that localize to the axoneme, spindle and cortical cytoskeleton nucleating structures. j, Localization of proteins identified as upregulated at the protein level in particular cell cycle stages by Crozier et al.43.
