Fig. 4: Expansive ANME-1 mobilome includes 16 undescribed viral families and structurally predicted MCPs.
From: Evolutionary diversification of methanotrophic ANME-1 archaea and their expansive virome
a, Histograms showing the number of CRISPR spacers from South Pescadero and Guaymas basin metagenomes matching the South Pescadero ANME-1 MGEs. b, Gene sharing network of diverse ANME-1 MGEs of different origins. c, ANME-1 MGEs, exhibited in the same network as b, are found to encompass major archaeal virus diversity and non-viral elements. Solid or open circles indicate viral assemblies with/without identifiable MCPs. In (b) and (c), dashed lines encircle five proposed viral families containing complete genome representatives. The proposed names of viral families (black) and orders (purple) are indicated in (c). d–f, Gene synteny of three proposed families of tailless icosahedral viruses targeting ANME-1. Different colours indicate 83 different protein groups. Grey shading denotes singletons. The scale bar and precent identity shading are indicated in (f). g, Alphafold2-predicted structures of DJR MCPs in ANME-1 viruses shown in (d) and (e). Blue indicates β barrels, and red α helices. h, Maximum-likelihood analysis of proposed MCP families indicates their long evolutionary distances. i, Maximum-likelihood analysis of PolB found in different clades of the tailless Chaacviruses targeting ANME-1 archaea are related to two clades of spindle-shaped Wyrdviruses targeting Asgard archaea. SJR, single jelly-roll.
