Extended Data Fig. 5: Phenotypic changes to CD4⁺ T cells after CD8⁺ depletion.

Longitudinal flow cytometry analysis after CD8⁺ T cell depletion in Pre-infection CD8⁺ depletion group (n = 8 macaques, magenta line), Pre-ART CD8⁺ depletion group (n = 8 macaques, teal line), and control group (n = 5 macaques, black line). Blue triangle indicates SIVmac239M infection, red triangle indicates CD8⁺ T cell depletion prior to infection, orange triangle indicates CD8⁺ T cell depletion prior to ART initiation. Grey box represents ART. Lines on each graph represents individual animals in each study group and indicated in key under each column of graphs. Percentage of CD28⁺CD95^- T_N, CD28⁺CD95⁺ T_CM, and CD28⁻CD95⁺ T_EM CD4⁺ T cells in peripheral blood (a-c,g) and lymph nodes (d-f,h). For Figures g, h: Data are mean ± s.e.m. Kruskal-Wallis test was used to compare values between the groups. * p value less than 0.05; ** p value less than 0.01.