Extended Data Fig. 2: Distributions and transduction efficiencies of the most enriched mutations in the T7 and the ΦSG-JL2 tail fibre displaying hybrid T7 bacteriophage particles when selected on E. coli ΔwaaR model strain.
a, The mutant T7 gp17 HRDRs usually carry specific combinations of mutations, 28% of which have been described as adaptive mutations previously. Heatmap representing the number of cases a mutation occurs in the 50 sequenced T7 phage tail HRDRs. Adaptive mutations according to (Huss et al.28) are indicated with a red dot. The frequent combination of specific adaptive mutations indicates the potential of DIvERGE to find host-specificity altering mutations with high efficiency. Data available in Supplementary Table 4. b,c, Distribution of detected mutations across the mutagenized phage tail fibre genes Escherichia phage T7 gp17 and Salmonella phage ΦSG-JL2 gp17. Predicted HRDRs are distinguished via colorized regions as in (Yehl et al.29) with the T3 bacteriophage. d, Transduction efficiencies of the mutant T7 (grey) and ΦSG-JL2 (yellow) phage tails as compared to their wild type counterparts with E. coli K12 BW25113 ΔtrxAΔwaaR LPS deficient strain. Y axis shows the number of transduced cells in 1 mL. Centre and error bars represent mean and standard error (n = 3 biologically independent experiments). Note that we did not observe any enriched mutant Salmonella phage Vi06 gp43, indicating that Salmonella phage Vi06 tail fibre binds to a cell surface receptor other than LPS. Data available in Supplementary Table 4.
