Fig. 5: Hydrogenase expression and activity of Sphingopyxis alaskensis.
From: Molecular hydrogen in seawater supports growth of diverse marine bacteria
a, Growth curve of S. alaskensis grown on Difco 2216 Marine Broth. Cultures were tested for gas consumption and collected for RT–qPCR in exponential phase (17 h, OD600 = 0.66) and stationary phase (168 h, 4 d post ODmax). Data are presented as mean ± s.d., n = 3 biologically independent samples. b, Number of transcripts of the group 2a [NiFe]-hydrogenase large subunit gene (hucL; locus Sala_3198) as measured by RT–qPCR in exponential and stationary phase cultures of S. alaskensis. Mean ± s.d. of three biological replicates (averaged from two technical duplicates) per condition. The comparison is statistically significant based on an unpaired two-sided t-test (**P = 0.0062). c, H2 oxidation by exponential and stationary phase cultures of S. alaskensis. Mean ± s.d. of three biological replicates, with media-only vials monitored as negative controls. Dotted line shows the atmospheric concentration of hydrogen (0.53 ppmv).
