Extended Data Fig. 2: Variability in Fionnbharth infection of LM14 and Δlsr2. | Nature Microbiology

Extended Data Fig. 2: Variability in Fionnbharth infection of LM14 and Δlsr2.

From: Mycobacterial nucleoid-associated protein Lsr2 is required for productive mycobacteriophage infection

Extended Data Fig. 2

(A) Cultures of M. smegmatis mc2155, M. smegmatis Δlsr2 and LM14 were grown from single colonies in septuplicate to show phenotypic variation of phage infection of M. smegmatis Δlsr2 and M. smegmatis LM14. Phage lysates were ten-fold serially diluted and spotted onto a single lawn of M. smegmatis mc2155, and seven for M. smegmatis Δlsr2 and LM14. (B) An Alamar Blue cell viability assay was performed infecting septuplicates of M. smegmatis mc2155, M. smegmatis Δlsr2 and LM14. The top three rows of each set contain uninfected controls, the middle six rows contain wells infected by BPsΔ33HTH and FionnbharthΔ45Δ47. The bottom three rows contain heat-killed controls of M. smegmatis mc2155, M. smegmatis Δlsr2 and LM14. (C) Box and whisker plots of the quantified relative fluorescence units of the Alamar Blue cell viability assay shown in panel B. Boxes are shaded according to strain as indicated in key. Boxes indicate Interquartile range, dark line inside boxes indicates the median, whiskers indicate minimum and maximum, 1.5 times the value of the interquartile range, outliers are indicated by dots. N = 7 biologically independent cultures.

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