Extended Data Fig. 7: Anti-Gr-1 effectively depletes neutrophils during infection.

(a) Flow cytometry plots displaying the gating strategy employed to identify neutrophils and monocytes upon anti-Gr-1 administration. Due to masking of Ly-6G and Ly-6C epitopes by anti-Gr-1, monocytes were identified as CCR2-GFP⁺ cells (green box) and neutrophils were identified as SSC high cells (pink boxes). Representative images of three independent experiments. (b) Frequency of monocytes in blood at day 5 post infection was determined by flow cytometry. Each symbol represents one mouse (n = 10-11). Lines represent median. Data from three independent experiments. Statistical analyses by Kruskal-Wallis test with Dunn’s post-test. * (p < 0.05), ** (p < 0.01), *** (p < 0.001), **** (p < 0.0001), NS (not significant, p > 0.05).