Extended Data Fig. 3: CCR2-deficient mice cannot control intestinal Yersinia.

(a) Quantification of total number of intestinal lesions at day 3 post infection. Each data point indicates one mouse (n = 9-11). Line represents median. Pooled from two independent experiments. (b) H&E-stained paraffin-embedded small-intestinal sections containing Peyer’s patches from WT and Ccr2^gfp/gfp mice at day 5 post infection. Dashed circle denotes area containing pyogranuloma or necrosuppurative lesion. Scale bars = 500 μm. Representative images from two independent experiments. (c) Bacterial burdens in small intestinal PG- and PG + tissue at day 3 post infection. Each data point represents mean CFU of 3-5 pooled punch biopsies from one mouse (n = 15-25). Lines represent geometric mean. Pooled data from four independent experiments. (d) Frequency of monocytes in blood at day 5 post infection. Each data point represents one mouse (n = 29-30). Lines indicate median. Pooled data from four independent experiments. (e) Bacterial burdens in small intestinal PG- and PG + tissue at day 5 post infection. Each data point represents the mean CFU of 3-5 pooled punch biopsies from one mouse (n = 21-30). Lines indicate geometric mean. Data pooled from four independent experiments. (f) Bacterial burdens in small intestinal PG- and PG + tissue at day 3 post infection. Each data point represents the mean CFU of 3-5 pooled punch biopsies from one mouse (n = 10-13). Lines indicate geometric mean. Pooled data from two independent experiments. (g) H&E-stained paraffin-embedded sections containing pyogranulomas from WT and Ccr2^gfp/gfp mice at day 3 post-infection. Dashed circle denotes area containing pyogranuloma. Scale bars = 100 μm. Representative images of one experiment. All statistical analyses by Mann-Whitney U test (two-tailed). * (p < 0.05), ** (p < 0.01), *** (p < 0.001), **** (p < 0.0001), NS (not significant, p > 0.05).