Extended Data Fig. 6: Functional CISSc production promotes stress-induced cell death.
From: Cytoplasmic contractile injection systems mediate cell death in Streptomyces
a/b. fLM (shown are representative images) was used to determine the ratio between live cells (cytoplasmic sfGFP) and total cells (membrane dye FM5-95) after growth in the presence of 10 µM CCCP (or 0.002 % DMSO as mock control) (a), or after exposure to UV light (b). S. coelicolor WT/sfGFP, ∆CIS/sfGFP and CIS-N5/sfGFP were grown in TSB for 48 h and treated with CCCP or DMSO for 90 min or were exposed to UV light for 10 min. Bars, 10 µm. The quantification for both experiments is shown in Fig. 4e/f.
