Fig. 2: MDMs from vsPWH have consistent levels of HIV DNA over time.
From: Monocyte-derived macrophages contain persistent latent HIV reservoirs
a, Ten vsPWH were assessed for HIV gag DNA, gag RNA and tat/rev RNA in isolated CD4 T cells (n = 10) and MDMs (n = 20), 6 donors were repeated 2–4 times. ****P < 0.0001, two-tailed unpaired t-test. b, HIV gag DNA in MDMs was assessed in 6 donors at multiple blood draws between 150–1,300 d apart. c, The number of CD4 T cells per million cells plated in MDM cultures, calculated using TCRβ RNA and CD4 percentages in whole blood; n = 15, 3 donors were repeated 2–3 times, line indicates median. d, In a subset of individuals, HIV gag DNA was assessed in monocytes and MDM from the same blood draw; n = 4. e, Monocytes and CD4 T cells were isolated from 30 vsPWH and assessed for HIV proviral DNA using IPDA. Intact, 3’ defective, 5’ defective and total proviral genome levels per million cells were compared between cell types; intact **P = 0.0014, 3’ del *P = 0.03, 5’ del ***P = 0.0005, total **P = 0.006, two-tailed unpaired t-test. f, Comparison of intact genome levels in a subset of participants that had detectable intact genomes in both CD4 and monocytes; n = 12, two-tailed paired t-test. NS, not significant. Each datapoint represents data from a specific participant, circles are CD4 data, squares are monocytes or MDM data and lines represent medians.
