Fig. 3: MDMs from vsPWH have reactivatable reservoirs that can be induced over time and stratify with HIV DNA burden.
From: Monocyte-derived macrophages contain persistent latent HIV reservoirs
a, Ten vsPWH were assessed for reactivatable reservoirs in CD4 T cells and MDMs isolated from the same blood draw using the cell-specific CD4 and MDM QVOAs. b, Four participants returned for a second visit 150–280 d after the first visit. All participants had repeat MDM-QVOA completed and one participant also had a repeat CD4-QVOA completed (CP36, orange circle). Two of the 4 participants returned for a third follow-up visit 1,174 and 1,502 d after their first visit to repeat the MDM-QVOA. c, Average HIV gag RNA copies per million cells plated in the CD4 and MDM QVOA; n = 9 CD4 and n = 10 MDM, not significant via unpaired t-test. d, Participants with detectable IUPM values in MDM-QVOA had higher levels of HIV gag DNA compared with those with undetectable IUPM values; n = 9 detectable and n = 5 undetectable, two-tailed unpaired Student’s t-test P = 0.0122. e, MDM DNA levels positively correlated with MDM IUPM values; simple linear regression R2 = 0.48 and P = 0.04. f,g, Comparing immune cell percentages in blood from participants with detectable (n = 10) and undetectable (n = 5) IUPM values in MDM QVOA; total monocytes (TLR2+/CD3−) and CD4 T cells (f), and monocyte subsets (g).
