Extended Data Fig. 2: Comparison of whole blood, PBMC, negatively selected monocyte subsets.
From: Monocyte-derived macrophages contain persistent latent HIV reservoirs
Whole blood (A), PBMC (B) and negatively selected monocytes (C) from one representative subject used to show that negative selection results in less CD4 contamination in cells plated and similar monocyte subset percentages. Cells are first gated on TLR2 expression, monocytes (TLR2+) are further gated by CD14 and CD16 expression, classical (CD14 + CD16−), intermediate (CD14 + CD16+) and non-classical (CD14-CD16+). Lymphocytes (TLR2-) are gate based on CD3 and CD4 expression, CD4 T cells (CD3 + CD4+).
