Extended Data Fig. 2: Identification of SHISA9-regulated pathways in brain during HSV-1 infection.
From: Modulation of virus-induced neuroinflammation by the autophagy receptor SHISA9 in mice
(a) Global gene expression in WT and Shisa9−/− mice brains are presented as heat map. Microarray analysis for total RNA was performed for WT and Shisa9−/− mice brain before (day 0, HSV-1 uninfected, UI) and after intracranial infection with HSV-1(1 × 102 PFU) (day 3; day 7), results are presented as log2 (TPM + 1). (b) Relative expression of genes related to ‘Chemokine’, ‘Pattern Recognition Receptor Signaling’, ‘NF-κB’ and ‘Lysosome’ pathways in WT mice and Shisa9−/− mice, results are presented as mean of TPM from RNA seq analysis at day 0 (UI), 3, 7 after intracranial infection with HSV-1 (1 × 102 PFU). (c and d) KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis of up-regulated genes in Shisa9−/− mice compared with WT mice at day 3 (c), and day 7 (d) after intracranial infection with HSV-1(1 × 102 PFU). TPM, transcripts per million.
