Extended Data Fig. 7: Comparison of the PaFtsWIQBL cryo-EM structure with a RodA-PBP2 crystal structure and AlphaFold 2 structure prediction.
From: Cryo-EM structure of the bacterial divisome core complex and antibiotic target FtsWIQBL
Comparison of the cryo-EM structure PaFtsWIQBL (a), the Thermus thermophilus RodA-PBP2 crystal structure (PDB: 6PL5, b) and the AlphaFold 2 prediction of PaFtsWIQBL (c). All three structures were aligned on FtsW/RodA. The FtsQPOTRA and FtsQTM of the AlphaFold2 model were removed for clarity. The FtsI/PBP2 periplasmic domains show a large 130° lateral rotation between the P. aeruginosa FtsWI and T. thermophilus RodA-PBP2 models (a-b). The rotation was measured around an axis perpendicular to the membrane plane and intersecting the FtsW active site. The distance between both active sites in FtsI (S294) and PBP2 (S308) is 125 Å. A 30° vertical rotation of the periplasmic FtsI domains is visible between the cryo-EM and AlphaFold2 models of PaFtsWIQBL (a-c). The angle was measured between the FtsW active site (D275) and the FtsI active sites (S294). The distance between the FtsI active sites in the cryo-EM and AlphaFold2 models is 46 Å.
